DNA fingerprinting: a quality control case study for human biospecimen authentication.

نویسندگان

  • Olga A Kofanova
  • William Mathieson
  • Gerry A Thomas
  • Fotini Betsou
چکیده

This case study illustrates the usefulness of the DNA fingerprinting method in biobank quality control (QC) procedures and emphasizes the need for detailed and accurate record keeping during processing of biological samples. It also underlines the value of independent third-party assessment to identify points at which errors are most likely to have occurred when unexpected results are obtained from biospecimens.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Authentication and quality control of some polyherbal oils used in Persian Traditional Medicine (PTM)

Background and objectives: Traditional polyherbal oils are still in use in Persian Traditional Medicine (PTM). Most of these formulations are prepared via traditional procedures such as maceration of herbs in oils or evaporating aqueous herbal extracts in boiling or heating oils as the vehicle. Thus, their quality control, standardization and authentication are real challenges ...

متن کامل

Comparison of Two Multiplex PCR Systems for Meat Species Authentication

Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication.  Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...

متن کامل

National Cancer Institute Biospecimen Evidence-Based Practices: a novel approach to pre-analytical standardization.

Variable biospecimen collection, processing, and storage practices may introduce variability in biospecimen quality and analytical results. This risk can be minimized within a facility through the use of standardized procedures; however, analysis of biospecimens from different facilities may be confounded by differences in procedures and inferred biospecimen quality. Thus, a global approach to ...

متن کامل

Identification of Medicinal Mangrove Rhizophora apiculata Blume: Morphological, Chemical and DNA Barcoding Methods

"Kandal" (Rhizophora apiculata Blume) is a medicinal mangrove plant used to treat pain, inflammation and reduce blood glucose level in Southeast coast of India. Identification and revision of R. apiculata are largely based on morphological characteristics. Previously, we reported and filed a patent on anti-diabetic Glycosin from R. apiculata. Subsequently, for the quality and market authorizati...

متن کامل

Human biospecimen research: experimental protocol and quality control tools.

Human Biospecimen Research: Experimental Protocol and Quality Control Tools. Fotini Betsou, Rebecca Barnes, Thomas Burke, Domenico Coppola, Yvonne DeSouza, James Eliason, Barbara Glazer, David Horsfall, Cynthia Kleeberger, Sylvain Lehmann, Anil Prasad, Amy Skubitz, Stella Somiari, and Elaine Gunter (International Society for Biological and Environmental Repositories (ISBER) Working Group on Bio...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Biopreservation and biobanking

دوره 12 2  شماره 

صفحات  -

تاریخ انتشار 2014